Home Products Antibodies Primary Antibodies 50 142 28
50-142-28

CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), Functional Grade, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each 50-142-28-Each In Stock ₹ 23,998.85

50-142-28 - Each

₹ 23,998.85

In Stock

Quantity

1

Base Price: ₹ 23,998.85

GST (18%): ₹ 4,319.793

Total Price: ₹ 28,318.643

Antigen

CD11b (activation epitope)

Classification

Monoclonal

Concentration

1 mg/mL

Formulation

PBS with no preservative; pH 7.2

Gene Accession No.

P11215

Gene Symbols

ITGAM

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

3684

Content And Storage

4° C

Form

Liquid

Applications

Flow Cytometry, Functional Assay, Neutralization

Clone

CBRM1/5

Conjugate

Functional Grade

Gene

ITGAM

Gene Alias

antigen CD11b (p170); antigen CD11b (p170); macrophage antigen alpha polypeptide; CD11 antigen-like family member B; CD11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha M; integrin alpha-M; integrin subunit alpha M; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; leukocyte integrin alpha-M chain; LOC100351865; Ly-40; MAC1; Mac-1; Mac-1 alpha; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6; unnamed protein product

Host Species

Mouse

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

Description

  • Description: The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1
  • CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells
  • Through interactions with its ligands, Mac-1 participates in adhesive cell interactions
  • The epitope recognized by this mAb localizes to the I domain on the alpha chain of Mac-1 very close to the ligand binding site in a region that is widely exposed
  • CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1)
  • It should be noted that low level activation may occur during processing of freshly drawn blood
  • Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples
  • However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells
  • Applications Reported: The CBRM1/5 antibody has been reported for use in flow cytometric analysis
  • CBRM1/5 has also been reported in blocking of CD11b function
  • Applications Tested: The CBRM1/5 antibody has been tested by flow cytometric analysis of resting and activated normal human peripheral blood cells
  • This can be used at less than or equal to 1 μg per test
  • CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18)
  • The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3)
  • CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain
  • CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions
  • CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue
  • The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages
  • Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages
  • Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.