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50-145-98

CD314 (NKG2D) Monoclonal Antibody (CX5), Functional Grade, eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 50-145-98-Each-of-1 In Stock ₹ 20,114.00

50-145-98 - Each of 1

₹ 20,114.00

In Stock

Quantity

1

Base Price: ₹ 20,114.00

GST (18%): ₹ 3,620.52

Total Price: ₹ 23,734.52

Antigen

CD314 (NKG2D)

Classification

Monoclonal

Concentration

1 mg/mL

Formulation

PBS with no preservative; pH 7.2

Gene Accession No.

O54709

Gene Symbols

Klrk1

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

27007

Content And Storage

4° C

Form

Liquid

Applications

Flow Cytometry, Functional Assay, Neutralization

Clone

CX5

Conjugate

Functional Grade

Gene

Klrk1

Gene Alias

CD314; D12S2489E; D6H12S2489E; killer cell lectin like receptor K1; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; Klrk1; natural killer cell group 2D; natural killer cell receptor; NK cell receptor D; NK lectin-like receptor; Nkg2d; NKG2-D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor; NKLLR; Nkrp2; NKR-P2; NKR-P2, ortholog of human NKG2D

Host Species

Rat

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG1 κ

Description

  • Description: The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells
  • Mouse NKG2D binds to retinoic acid-inducible RAE-1alpha, -beta, -gamma, -delta, -ε and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules
  • NKG2D is expressed by all spleen and liver NK cells, NK1.1^+ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells
  • Applications Reported: The CX5 antibody has been reported for use in flow cytometric analysis
  • It has also been reported in blocking of binding of NKG2D to its ligands, RAE-1 and H60, and inhibition NKG2D-dependent NK cell-mediated cytotoxicity against NKG2D ligand-bearing tumors in vitro and in vivo
  • Applications Tested: The CX5 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that when using this antibody for staining the secondary must be Biotin anti-rat (cat 13-4813)
  • Other secondaries may give suboptimal staining
  • Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation
  • NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function
  • The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain
  • The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.