50-146-83

MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), Functional Grade, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

CAS Number: 28-14-8

Select a Size

Pack Size SKU Availability Price
Each of 1 50-146-83-Each-of-1 In Stock ₹ 13,172.00

50-146-83 - Each of 1

₹ 13,172.00

In Stock

Quantity

1

Base Price: ₹ 13,172.00

GST (18%): ₹ 2,370.96

Total Price: ₹ 15,542.96

Antigen

MHC Class I (H-2Db)

Classification

Monoclonal

Concentration

1 mg/mL

Formulation

PBS with no preservative; pH 7.2

Gene Accession No.

P01895, P01896, P01897, P01899, P01900, P14426, P14427

Gene Symbols

H2-D1

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

14964

Content And Storage

4° C

Form

Liquid

Applications

Control, Flow Cytometry, Functional Assay

Clone

28-14-8

Conjugate

Functional Grade

Gene

H2-D1

Gene Alias

H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein

Host Species

Mouse

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG2a κ

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Description

  • Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q
  • Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin
  • Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Storage and handling: Use in a sterile environment
  • Filtration: 0.2 μm post-manufacturing filtered
  • Purity: Greater than 90%, as determined by SDS-PAGE
  • Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
  • Aggregation: Less than 10%, as determined by HPLC
  • This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8
  • HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.