Granzyme B Recombinant Rabbit Monoclonal Antibody (BLR022E), Bethyl Laboratories

Description

• The recommended shelf life for this product is 1 year from date of receipt
• Application Note: For ICC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• For IHC, epitope retrieval with citrate buffer pH 6.0 is recommende Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells
• Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells
• Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells
• The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis
• Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis
• Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein
• Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells
• A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.