NFKBIA Monoclonal Antibody (OTI2F5), TrueMAB™, OriGene

Description

• IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity
• The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol
• The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus
• A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha
• These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses
• In humans, the gene is located on the q arm of chromosome 14
• Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB
• IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36
• IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines
• Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1
• The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.