50-194-3847
Phospho-IRS1 (Ser307)Rabbit anti-Human, Polyclonal, Rockland™
Manufacturer: Rockland Immunochemicals
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-194-3847-Each-of-1 | In Stock | ₹ 52,930.08 |
50-194-3847 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,930.08
GST (18%): ₹ 9,527.414
Total Price: ₹ 62,457.494
Antigen
Phospho-IRS1 (Ser307)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
IRS1
Gene Alias
G972R; HIRS-1; Insulin receptor substrate 1; IRS1; Irs-1; IRS1IRM; pp185
Host Species
Rabbit
Regulatory Status
RUO
Gene ID (Entrez)
3667
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Form
Liquid
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration
1 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Gene Accession No.
P35568
Gene Symbols
IRS1
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Related Products
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue
- Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein
- Reactivity with non-phosphorylated human IRS1 is minimal by ELISA
- A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100% homology with the immunizing sequence
- Partial reactivity is also expected against IRS1 from pig (94%), bovine (94%) and chicken (88%) sources
- Reactivity of this antibody with IRS1 from other species is unknown
- IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4)
- IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues
- An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo
- Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II
- Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade
- IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.