Histone H2A.XS139ph Rabbit anti-Human, Clone: RM224, Active Motif™
Manufacturer: Active Motif
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-199-3814-Each-of-1 | In Stock | ₹ 52,510.00 |
50-199-3814 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,510.00
GST (18%): ₹ 9,451.80
Total Price: ₹ 61,961.80
Antigen
Histone H2A.XS139ph
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
PBS with 1% BSA, 50% glycerol and 0.09% sodium azide; pH 7.5
Gene Accession No.
P16104
Gene Symbols
H2AX
Immunogen
A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139).
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Clone
RM224
Conjugate
Unconjugated
Gene
H2AX
Gene Alias
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2a.x; H2a/x; h2afx; H2AX; H2AX histone; Hist5-2ax; histone 5 protein 2ax; Histone H2A.X; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
3014
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- Description Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle
- In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX
- The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs
- The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner
- Structurally, H2A.x contains 143 amino acid residues
- Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs
- There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA
- The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA
- Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome
- Histone H2A.x contributes to histone-formation and therefore the structure of DNA
- Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.