Ly-6D Monoclonal Antibody (49-H4), PE, eBioscience™, Invitrogen™

Description

• Description: This 49-H4 monoclonal antibody reacts with mouse Ly-6D, which is also known as ThB
• This member of the Ly6 family of GPI-anchored cell surface glycoproteins is expressed on thymocytes and splenic B cells
• Moreover, common lymphoid progenitor cells that express Ly-6D have been shown to commit to the B cell lineage
• Expression of Ly-6D has also been associated with Rag-1 expression along with a reduced ability to differentiate into T or NK cells
• Finally, reports indicate that the level of Ly-6D expression varies in different mouse strains
• Applications Reported: This 49-H4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 49-H4 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• May act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development
• Marks the earliest stage of B-cell specification
• [UniProt]