AIRE Monoclonal Antibody (5H12), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-246-082-Each-of-1 | In Stock | ₹ 38,270.00 |
50-246-082 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,270.00
GST (18%): ₹ 6,888.60
Total Price: ₹ 45,158.60
Antigen
AIRE
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
Q9Z0E3
Gene Symbols
Aire
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
11634
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen)
Clone
5H12
Conjugate
Alexa Fluor 488
Gene
Aire
Gene Alias
Aire; AIRE protein; AIRE1; APECED; APECED protein; APECED protein homolog; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog; Autoimmune regulator; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy); autoimmune regulator AIRE1a; LOW QUALITY PROTEIN: autoimmune regulator; OTTHUMP00000109529; PGA1
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2c κ
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Description
- Description: This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE)
- This 58-kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs)
- However, expression of AIRE protein in the periphery remains unclear despite the detection of AIRE mRNA in the lymph node, lymphoid stromal cells, monocytes, and dendritic cells
- Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection
- Other biological functions for AIRE include chemokine expression and antigen presentation
- The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2
- Applications Reported: This 5H12 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections (IHC-F)
- Applications Tested: This 5H12 antibody has been tested by immunohistochemistry on fixed, frozen mouse thymus at less than or equal to 10 μg/mL
- It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest
- Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
- Filtration: 0.2 μm post-manufacturing filtered
- AIRE is a transcriptional regulator protein that binds to DNA as a dimer and tetramer, but not as a monomer, and may be involved in the regulation of gene expression
- A nuclear protein, AIRE is a 545 amino acid protein with an HSR domain, two PHD type zinc fingers, and a SAND domain, and may be present in soluble high-molecular-weight complexes
- It may form homodimer, homotetramer and also nuclear bodies and interacts with the transcriptional coactivator CBP and CREBBP
- Three isoforms of AIRE are reported and one of them is predicted to be a candidate for nuclear-mediated decay (NMD)
- AIRE is involved in immune regulation and is known to mediate E3 ubiquitin ligase activity
- Defects in AIRE are responsible for the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).