SCF Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-271-8457-Each-of-1 | In Stock | ₹ 1,75,552.50 |
50-271-8457 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,75,552.50
GST (18%): ₹ 31,599.45
Total Price: ₹ 2,07,151.95
Antigen
SCF
Classification
Polyclonal
Gene
Kitl
Gene Alias
710-712; blz; c-Kit ligand; Clo; cloud gray; Con; contrasted; CSF; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; Gb; grizzle-belly; hematopoietic growth factor KL; H-SCF; Kit ligand; Kitl; Kitlg; kit-ligand; KL-1; Mast cell growth factor; MGF; M-SCF; Processed kit ligand; SCF; SF; SHEP7; sKITLG; Sl; SLF; Soluble KIT ligand; Steel factor; steel factor/kit ligand; stem cell factor; stem cell factor KL-1; stem cell factor, CSF
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
17311
Form
Lyophilized
Applications
Western Blot, ELISA
Formulation
PBS with no preservative
Gene Accession No.
P20826
Gene Symbols
Kitl
Immunogen
E.coli-derived, 18.3 kDa Recombinant Murine SCF
Quantity
500 μg
Primary or Secondary
Primary
Target Species
Mouse
Isotype
IgG
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Description
- AA Sequence of recombinant protein: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine SCF
- Anti-Murine SCF-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect mSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine SCF (500-P71) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mSCF
- Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- Used in conjunction with compatible secondary reagents the detection limit for Recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P71BT-1MG will be provided as 2 x 500 μg Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways
- Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways
- SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines
- The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma
- At least two isoforms of SCF are known to exist.