50-271-8468
IL-10 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-271-8468-Each-of-1 | In Stock | ₹ 1,75,552.50 |
50-271-8468 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,75,552.50
GST (18%): ₹ 31,599.45
Total Price: ₹ 2,07,151.95
Antigen
IL-10
Classification
Polyclonal
Conjugate
Biotin
Gene
IL10
Gene Alias
CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
16153
Content And Storage
-20°C
Isotype
IgG
Applications
Western Blot, ELISA
Concentration
0.1-1.0 mg/mL
Formulation
PBS with no preservative
Gene Accession No.
P18893
Gene Symbols
IL10
Immunogen
E.coli-derived Recombinant Murine IL-10
Quantity
500μg
Primary or Secondary
Primary
Target Species
Mouse
Form
Lyophilized
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Description
- AA Sequence of recombinant protein: MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-10
- Anti-Murine IL-10-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect mIL-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-10 (500-P60) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIL-10
- Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P60BT-1MG will be provided as 2 x 500 μg IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes
- This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages
- It also enhances B cell survival, proliferation, and antibody production
- Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway
- Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract
- Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis
- Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.