CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), FITC, eBioscience™, Invitrogen™

Description

• Description: The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily
• This family is characterized by the presence of four hydrophobic domains spanning the cell membrane and short N- and C-terminal cytoplasmic domains
• CD9 is expressed by several cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts and neuroblasts
• On T cells, CD9 functions as a co-stimulatory molecule on naive T cells
• Furthermore, CD9 is expressed in oocytes, and CD9-deficiency results in sterility caused by defective gamete fusion
• In mouse macrophages, CD9 functionally associates with FcgammaRs to modify signals for phagocytosis and inflammatory responses
• In mouse B cells, it was discovered that CD9 is a marker for marginal zone B cells, B1 cells, and plasma cells
• In dendritic cells, recently it was demonstrated that CD9 facilitates the association of heterologous MHC II molecules
• The level of CD9 expression is subject to donor variability
• Applications Reported: This eBioKMC8 (KMC8) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioKMC8 (KMC8) antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells
• This can be used at less than or equal to 1 µg per test
• A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system
• CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times
• In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa)
• CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation
• CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis
• CD9 is also considered as metastasis suppressor in solid tumors.