Midkine Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 500P171BT1-Each-of-1 | In Stock | ₹ 3,51,105.00 |
500P171BT1 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,51,105.00
GST (18%): ₹ 63,198.90
Total Price: ₹ 4,14,303.90
Antigen
Midkine
Classification
Polyclonal
Conjugate
Biotin
Gene
Mdk
Gene Alias
amphiregulin-associated protein; ARAP; FLJ27379; MDK; Mek; Midgestation and kidney protein; midkine; midkine (neurite growth-promoting factor 2); MK; MK1; NEGF2; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; Retanoic acid-responsive protein; retinoic acid inducible factor; retinoic acid-induced differentiation factor; retinoic acid-responsive protein
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
4192
Content And Storage
-20°C
Applications
ELISA, Western Blot
Concentration
0.1-1.0 mg/mL
Formulation
PBS with no preservative
Gene Accession No.
P21741
Gene Symbols
Mdk
Immunogen
E.coli-derived Recombinant Human Midkine
Quantity
2 x 500 μg
Primary or Secondary
Primary
Target Species
Human
Form
Lyophilized
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Description
- AA Sequence of recombinant protein: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Midkine
- Anti-Human Midkine-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Midkine (500-P171) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMidkine
- Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMidkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P171BT-1MG will be provided as 2 x 500 μg Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis
- It promotes angiogenesis, cell growth, and cell migration
- Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis.