500P84BT25
BDNF Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 500P84BT25-Each-of-1 | In Stock | ₹ 20,203.00 |
500P84BT25 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 20,203.00
GST (18%): ₹ 3,636.54
Total Price: ₹ 23,839.54
Antigen
BDNF
Classification
Polyclonal
Conjugate
Biotin
Gene
BDNF
Gene Alias
abrineurin; ANON2; anorexia BDNF; Bdnf; BDNF precursor form; bdnf protein; brain derived neurothrophic factor; brain derived neurotrophic factor; brain-derived neurotrophic factor; BULN2; H-BDNF; neurotrophin; ProBDNF
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
12064, 24225, 627
Content And Storage
-20°C
Applications
ELISA, Western Blot
Concentration
0.1-1.0 mg/mL
Formulation
PBS with no preservative
Gene Accession No.
P21237, P23363, P23560
Gene Symbols
BDNF
Immunogen
E.coli-derived Recombinant Human/Murine/Rat BDNF
Quantity
25 μg
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Lyophilized
Related Products
Description
- AA Sequence of recombinant protein: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BDNF
- Anti-Human/Murine/Rat BDNF-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect Human BDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BDNF (500-P84) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BDNF
- Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P84BT-1MG will be provided as 2 x 500 μg BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family
- BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF
- Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form
- BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons
- Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated
- BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation
- BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain
- Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.