Caspase-3 Antibody - (active/cleaved), Novus Biologicals™

Caspase-3

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot

Unconjugated

Rabbit

Apoptosis, Cancer, Caspases, Phospho Specific

Neat whole antisera with 0.05% Sodium Azide

836

This Caspase-3 Antibody - (active/cleaved) was developed against catalytically active human caspase-3 protein.

Primary

Store at -20C. Avoid freeze-thaw cycles.

31.7 kDa

Western Blot 1:1000-1:2000, Flow Cytometry reported in scientific literature (PMID 28287161), Immunohistochemistry, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 29963272), Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen reported in scientific literature (PMID 29864441), Immunoblotting reported in scientific literature (PMID 27912032), Flow (Intracellular) reported in scientific literature (PMID 24804954)

Polyclonal

Antisera

RUO

Human, Mouse, Rat, Gerbil

Apopain, apoptosis-related cysteine protease, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama

CASP3

IgG

Unpurified

Preferentially detects active caspase-3 (large subunit: ∼14-21 kDa, and small subunit: ∼10 kDa). However, it may also detect pro-caspase-3 (∼32 kDa) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3.