5078345

LC3A Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 265 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

LC3A

Dilution

Western Blot 2.0 ug/ml, Simple Western 1:50, Chromatin Immunoprecipitation reported in scientific literature (PMID 33035707), Flow Cytometry reported in scientific literature (PMID 24419333), ELISA reported in scientific literature (PMID 20930550), Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:100-1:300. Use reported in scientific literature (PMID 21545732), Immunoprecipitation 20 ug/ 500 ug of lysate, Immunohistochemistry-Paraffin 1:200-1:400. Use reported in scientific literature (PMID 26571030), Immunohistochemistry-Frozen, Immunoblotting reported in scientific literature (PMID 28253371), Southern Blot, Immunohistochemistry (Whole-Mount) reported in scientific literature (PMID 31783118), Chromatin Immunoprecipitation (ChIP)

Classification

Polyclonal

Host Species

Rabbit

Research Discipline

Autophagy, Cancer, Cellular Markers, Cytoskeleton Markers, Hypoxia, Macroautophagy, Signal Transduction

Formulation

PBS with 0.02% Sodium Azide

Gene Alias

ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, lc3-i, ii, MAP1A/1B light chain 3 A, MAP1ALC3MAP1A/MAP1B LC3 A, MAP1BLC3MAP1A/MAP1B light chain 3 A, microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A

Gene Symbols

MAP1LC3A

Isotype

IgG

Purification Method

Affinity Purified

Test Specificity

This antibody detects both LC3A and LC3B.

Concentration

1.0 mg/mL

Applications

Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In Situ Hybridization (ISH)

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Amphibian, Canine, Fish, Plant, Zebrafish

Gene Accession No.

Q9GZQ8

Gene ID (Entrez)

84557

Immunogen

A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [Uniprot: Q9H492].

Primary or Secondary

Primary

Content And Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

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Description

  • LC3A Polyclonal specifically detects LC3A in Human, Mouse, Rat, Amphibian, Canine, Fish, Plant, Zebrafish samples
  • It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Southern Blot, Immunohistochemistry Whole-Mount.