TRAF-2 Antibody, Novus Biologicals™

TRAF-2

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Unconjugated

Rabbit

Apoptosis, Cancer, Cytokine Research, Zinc Finger

Whole antisera with 0.05% Sodium Azide

7186

A synthetic peptide corresponding to amino acids 87-106 (SSSAFPDNAARREVESLPAV) of human TRAF2 was used as immunogen, GenBank no. NP_066961.2. This peptide sequence is 100% conserved in mouse and rat TRAF2. The peptide immunogen sequence is 100% conserved in multiple human TRAF2 GenBank accession numbers. These TRAF2 accession numbers are of varying amino acid (aa) lengths and include: 1. NP_066961.2 = 501 aa. 2. CAI12705.1 =121 aa. 3. CAI12704.1 = 422 aa. 4. CAI15104.1 = 193 aa. 5. BAD96681.1 = 205 aa.

Primary

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western Blot 1/1000 - 1/200, Immunohistochemistry, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 33471802), Immunoprecipitation, Immunohistochemistry-Paraffin 1/1000 - 1/500, Immunohistochemistry-Frozen Use at an assay dependent dilution

Polyclonal

Antisera

RUO

Human, Mouse, Rat

E3 ubiquitin-protein ligase TRAF2, EC 6.3.2.-, MGC:45012, TNF receptor-associated factor 2, TRAP3TRAP, tumor necrosis factor type 2 receptor associated protein 3, Tumor necrosis factor type 2 receptor-associated protein 3

TRAF2

IgG

Unpurified

The gene encoding TRAF2 produces, by alternative splicing, 15 different transcripts, all with introns, putatively encoding 15 different protein isoforms. These isoforms are of varying amino acid lengths. Therefore the molecular weight observed by western blot may vary, depending on the isoform expressed. Users are encouraged to use the peptide immunogen sequence information provided in the antigen section to gain additional information about the potential TRAF2 isoforms detected by the antibody.