CD294 (CRTH2) Monoclonal Antibody (No3m1scz), Alexa Fluor™ 647, eBioscience™, Invitrogen™

Description

• Description: This clone recognizes mouse CD294, also known as Prostaglandin D2 receptor 2 (PD2R2), G-protein coupled receptor 44 (GPR44) or Chemoattractant Receptor-Homologous Molecule Expressed On TH2 Cells (CRTH2)
• Although the clone works on unfixed mouse CD294-transfected cells, we recommend fixing and permeabilizing primary cells
• Applications Reported: This No3m1scz antibody has been reported for use in flow cytometric analysis
• Applications Tested: This No3m1scz antibody has been tested by flow cytometric analysis of mouse lysed whole blood cells using Protocol A: Two-step protocol for intracellular (cytoplasmic) proteins
• This allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins
• Use of Protocol B: One-step protocol for intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins
• Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to Clone Performance Following Fixation/Permeabilization located in the Best Protocols Section under the Resources tab online)
• All Protocols can be found in the Staining Intracellular Antigens for Flow Cytometry Protocol located in the Best Protocols Section under the Resources tab online
• CRTH2 (GPR44) is a chemoattractant receptor-homologous molecule expressed on Th2 cells that is an identified receptor for the prostaglandin D2 (PGD2) and prostaglandin D receptor
• CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses
• However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3
• This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo
• CRTH2 is also a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells
• CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells
• Two transcripts have been isolated: a 3.5 kb transcript expressed primarily in the thalamus, frontal cortex, pons and hippocampus; and a 3.4 kb transcript expressed in fetal liver, leukocytes and thymus
• CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions
• At least two different isoforms of CRTH2 are known to exist
• Single nucleotide polymorphisms in the 3' UTR of the CRTH2 gene have been associated with asthma susceptibility.