GFAP Monoclonal Antibody (2.2B10), Alexa Fluor™ 647, eBioscience™, Invitrogen™
Manufacturer: eBioscience
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 51-979-282-Each-of-1 | In Stock | ₹ 37,380.00 |
51-979-282 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,380.00
GST (18%): ₹ 6,728.40
Total Price: ₹ 44,108.40
Antigen
GFAP
Classification
Monoclonal
Conjugate
Alexa Fluor 647
Gene
GFAP
Gene Alias
6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
Host Species
Rat
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
14580, 24387, 2670, 281189
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen)
Clone
2.2B10
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P03995, P14136, P47819, Q28115
Gene Symbols
GFAP
Immunogen
Enriched bovine glial filaments
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Bovine, Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG2a κ
Related Products
Description
- Description: This 2.2B10 monoclonal antibody recognizes human, mouse, rat, and bovine glial fibrillary acid protein (GFAP)
- The immunogen used to generate this antibody was enriched bovine glial filaments
- Applications Reported: This 2.2B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This 2.2B10 antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824) and protocol
- Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
- This may be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family
- GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves
- In addition, neural stem cells strongly express GFAP
- Antibodies to GFAP are very useful as markers of astrocytic cells
- In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP
- GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes
- GFAP is used as a marker to distinguish astrocytes from other glial cells during development
- Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system
- Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.