CD45R (B220) Monoclonal Antibody (RA3-6B2), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 53-045-280-Each-of-1 | In Stock | ₹ 10,591.00 |
53-045-280 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 10,591.00
GST (18%): ₹ 1,906.38
Total Price: ₹ 12,497.38
Antigen
CD45R (B220)
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P06800, P08575
Gene Symbols
PTPRC
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
19264, 5788
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Clone
RA3-6B2
Conjugate
Alexa Fluor 488
Gene
PTPRC
Gene Alias
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen
Host Species
Rat
Quantity
25 μg
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG2a κ
Related Products
Description
- The RA3-6B2 MAb reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule
- CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells
- However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen
- CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family
- PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation
- CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains
- CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling
- CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
- CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains
- Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
- In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
- CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
- Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.