CD195 (CCR5) Monoclonal Antibody (NP-6G4), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each | 53-195-642-Each | In Stock | ₹ 28,626.85 |
53-195-642 - Each
In Stock
Quantity
1
Base Price: ₹ 28,626.85
GST (18%): ₹ 5,152.833
Total Price: ₹ 33,779.683
Antigen
CD195 (CCR5)
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P51681
Gene Symbols
CCR5
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
1234
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
NP-6G4
Conjugate
Alexa Fluor 488
Gene
CCR5
Gene Alias
AM4-7; C-C chemokine receptor type 5; C-C CKR-5; C-C motif chemokine receptor 5 (gene/pseudogene); C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5; Ccr5; CCR-5; CD195; CD195 antigen; chemokine (C-C motif) receptor 5; chemokine (C-C) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; Cmkbr5; HIV-1 fusion coreceptor; IDDM22; MIP-1 alpha receptor
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The NP-6G4 monoclonal antibody reacts with human CD195 (CCR5), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1a, MIP-1b and RANTES
- Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells
- CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120
- Applications Reported: This NP-6G4 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This NP-6G4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- For optimal performance, it is recommended that staining with NP-6G4 be done at 37°C for 1 hour in Flow Cytometry Staining Buffer (Product # 00-4222-26)
- This may be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells
- Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors
- Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells
- CCR5 is required for the infection by HIV-1, HIV-2, and SIV
- The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1
- CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells
- The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites
- CCR5 messenger RNA is expressed in lymphoid organs and monocytes.