O-GlcNAc Monoclonal Antibody (RL2), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 53-979-341-Each-of-1 | In Stock | ₹ 16,109.00 |
53-979-341 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,109.00
GST (18%): ₹ 2,899.62
Total Price: ₹ 19,008.62
Antigen
O-GlcNAc
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
O15294
Gene Symbols
OGT
Immunogen
Rat liver nuclear envelopes
Quantity
25 Tests
Primary or Secondary
Primary
Target Species
Chemical
Product Type
Antibody
Isotype
IgG1 κ
Applications
Flow Cytometry
Clone
RL2
Conjugate
Alexa Fluor 488
Gene
OGT
Gene Alias
GlcNAc; HINCUT-1; HRNT1; O-GLCNAC; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; OGT; O-linked N-acetylglucosamine (GlcNAc) transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
Host Species
Mouse
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
8473
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Target Molecule
Chemical
Related Products
Description
- Description: This RL2 monoclonal antibody recognizes proteins with O-N-acetylglucosamine (O-GlcNAc) glycosylation
- It was originally developed by immunizing mice with the rat liver nuclear envelopes containing nuclear pore complexes
- The RL2 clone has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and flow cytometry in a wide variety of mammalian cells
- Applications Reported: This RL2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This RL2 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol
- Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online
- This may be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues
- Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm
- They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins
- O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.