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53-985-280

IRF8 Monoclonal Antibody (V3GYWCH), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 53-985-280-Each-of-1 In Stock ₹ 14,685.00

53-985-280 - Each of 1

₹ 14,685.00

In Stock

Quantity

1

Base Price: ₹ 14,685.00

GST (18%): ₹ 2,643.30

Total Price: ₹ 17,328.30

Antigen

IRF8

Classification

Monoclonal

Concentration

0.5 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P23611, Q02556

Gene Symbols

Irf8

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

15900, 3394

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

V3GYWCH

Conjugate

Alexa Fluor 488

Gene

Irf8

Gene Alias

AI893568; H-ICSBP; Icsbp; Icsbp1; IMD32A; IMD32B; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; Irf8; IRF-8; Myls; transcription factor ICSBP

Host Species

Mouse

Quantity

25μg

Primary or Secondary

Primary

Target Species

Human, Mouse

Product Type

Antibody

Isotype

IgG1 κ

Description

  • Description: The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP)
  • IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC)
  • Specifically, IRF8 is essential in the development and differentiation of plasmacytoid DC (pDC) and CD8a+ DC
  • IRF8-/- mice are deficient in both pDC and CD8a+ DC populations
  • CD8a- DC are present in normal numbers in IRF8-/- mice but fail to mature upon Toll-like receptor signaling and are functionally impaired in response to in vivo microbial stimulation
  • IRF8 deficiency does not affect the frequency or viability of DC precursors and as a result retroviral IRF8 transduction restores pDC development from DC progenitors in IRF8-/- mice
  • Applications Reported: This V3GYWCH antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This V3GYWCH antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
  • This may be used at less than or equal to 0.25 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
  • IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor
  • It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes
  • IRF8 plays a negative regulatory role in cells of the immune system
  • It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells
  • This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes
  • Mutations in the gene can result in immunodeficiencey 32A and 32B.