5325773

Mouse anti-HSP10/EPF, Clone: M1.2, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

HSP10/EPF

Concentration

0.92 mg/mL

Applications

Western Blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Core ESC Like Genes, Membrane Trafficking and Chaperones, Ovarian Carcinoma Cell Markers, Stem Cell Markers

Formulation

Liquid. In PBS containing 0.1mM PMSF. with No Preservative

Gene ID (Entrez)

3336

Immunogen

A 16 residue synthetic MAP peptide KKDDKGQQLPFEVQVG based on C. trachomatis cpn10 (residues 54-69).

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone

M1.2

Dilution

Western Blot 0.25 ug/ml, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 1:10-1:500

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Bacteria

Gene Alias

10 kDa chaperonin, 10 kDa heat shock protein, mitochondrial, Chaperonin 10, CPN10HSP10, Early-pregnancy factor, EPF, GROES, heat shock 10kD protein 1 (chaperonin 10), heat shock 10kDa protein 1 (chaperonin 10), Hsp10

Gene Symbols

HSPE1

Isotype

IgG2a

Purification Method

Protein G purified

Test Specificity

This affinity purified detects a 15 kDa protein, corresponding to the apparent molecular mass of cpn10 on SDS-PAGE immunoblots, in C. trachomatis infected cells. This will not react with the human or mouse cpn10 homolog nor with E. coli GroES.

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Description

  • HSP10/EPF Monoclonal specifically detects HSP10/EPF in Bacteria samples
  • It is validated for Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.