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5349254

MilliporeSigma™ anti-CD4 PE-Cy7, Clone: RM4-5,

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD4

Applications

Flow Cytometry

Conjugate

PE-Cyanine7

Host Species

Rat

Research Discipline

Inflammation, Immunology

Formulation

Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.

Gene Symbols

Cd4

Isotype

IgG2a κ

Purification Method

Purified by size-exclusion chromatography

Clone

RM4-5

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Mouse

Gene Accession No.

P06332

Immunogen

Proprietary immunogen

Primary or Secondary

Primary

Content And Storage

2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze

Description

  • Specifically detects CD4 Clone: RM4-5 in Mouse samples, and it is validated for Flow Cytometry The RM4-5 antibody reacts with mouse CD4, a 55kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells
  • The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation
  • CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression
  • If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will 'compete' for binding, i.e
  • RM4-5 antibody is able to block GK1.5 antibody binding to cells
  • In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.