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5349354

MilliporeSigma™ anti-CD8a FITC, Clone: 53-6.7,

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD8a

Applications

Flow Cytometry

Conjugate

FITC

Host Species

Rat

Research Discipline

Inflammation, Immunology

Formulation

Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.

Gene Symbols

CD8a

Isotype

IgG2a κ

Purification Method

Purified by size-exclusion chromatography

Clone

53-6.7

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Mouse

Gene Accession No.

P01731

Immunogen

Proprietary immunogen

Primary or Secondary

Primary

Content And Storage

2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze

Description

  • Specifically detects CD8a Clone: 53-6.7 in Mouse samples, and it is validated for Flow Cytometry The 53-6.7 antibody reacts with the 32-34kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha
  • CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta
  • CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules
  • The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions
  • CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.