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5377896

CD62L Mouse anti-Human, BB515, Clone: DREG-56, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD62L

Applications

Flow Cytometry

Conjugate

Brilliant Blue 515 (BB515)

Regulatory Status

RUO

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Isotype

IgG1 κ

Purification Method

Affinity Purified

Description

SELL; L-selectin; LSEL; LAM-1; LECAM-1; LEU8; LNHR; MEL-14; PLNHR; TQ-1

Clone

DREG-56

Classification

Monoclonal

Host Species

Mouse

Target Species

Human

Immunogen

Supernatant from PMA-activated Human Peripheral Blood Leukocytes

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Description

  • The DREG-56 monoclonal antibody specifically binds to CD62L
  • CD62L is a 76-95 kDa glycoprotein that is also referred to as L-selectin or LECAM-1
  • CD62L is expressed on neutrophils, monocytes, T- and B-lymphocyte subsets and NK cells
  • The DREG-56 antibody recognizes the same antigen as LAM-1, and specifically inhibits >90% of binding of human lymphocytes to high endothelial venules (HEV) in frozen sections of peripheral, but not mucosal lymphoid tissue
  • It thus defines L-selectin as a human lymphocyte homing receptor for peripheral lymph node HEV
  • The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences
  • With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter)
  • This dye provides a much brighter alternative to FITC with less spillover into the PE detector.