5377933
CD25 Mouse anti-Human, APC-R700, Clone: 2A3, BD
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD25
Applications
Flow Cytometry
Conjugate
APC-R700
Regulatory Status
RUO
Gene ID (Entrez)
100
Isotype
IgG1 κ
Purification Method
Affinity Purified
Description
IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
Clone
2A3
Classification
Monoclonal
Host Species
Mouse
Target Species
Human
Immunogen
Human Phytohemagglutinin-activated T Cells
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Description
- The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL- 2Rα)
- CD25 associates with CD122 (IL-2Rβ chain) and CD132 (common γchain or γc) to form the high-affinity signal-transducing IL -2R complex
- CD25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including CD4+CD25+ regulatory T cells and memory T cells
- CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes
- High levels of CD25 can be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102
- CD25 can also be expressed by activated B cells and macrophages
- Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes
- This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700
- APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness
- This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).