Human B7-H3 MAb (Clone 185504), R&D Systems™
Mouse Monoclonal Antibody has been used in 5 publications
Manufacturer: Fischer Scientific
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Antigen
B7-H3/CD276
Concentration
LYOPH
Applications
Western Blot, Flow Cytometry, CyTOF, KnockDown
Conjugate
Unconjugated
Regulatory Status
RUO
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias
B7H3, B7H34Ig-B7-H3, B7-H3B7 homolog 3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule
Gene Symbols
CD276
Isotype
IgG1
Purification Method
Protein A or G purified from hybridoma culture supernatant
Content And Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone
185504
Dilution
Western Blot 2 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, CyTOF-ready, Knockout Validated
Classification
Monoclonal
Host Species
Mouse
Target Species
Human
Gene Accession No.
NP_079516
Gene ID (Entrez)
80381
Immunogen
Mouse myeloma cell line NS0-derived recombinant human B7-H3 Leu29-Pro245 Accession # NP_079516
Primary or Secondary
Primary
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity
Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) B7-H1, rhB7-H2, rhB7-1, rhB7-2, or recombinant mouse PD-L2 is observed.
Related Products
Description
- B7-H3/CD276 Monoclonal specifically detects B7-H3/CD276 in Human samples
- It is validated for Western Blot, Flow Cytometry, CyTOF-ready, Knockout Validated.