5379929

Human/Mouse IGF-I R/IGF1R Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 17 publications

Manufacturer: Fischer Scientific

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Antigen

IGF-I R

Dilution

Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-25 ug/mL, Neutralization, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification

Monoclonal

Host Species

Mouse

Target Species

Human, Mouse

Gene Accession No.

P08069

Gene ID (Entrez)

3480

Immunogen

S. frugiperdainsect ovarian cell lineSf21-derived recombinant human IGF-I R Glu31-Asn932 Accession # P08069

Primary or Secondary

Primary

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity

Detects human IGF-I R in sandwich ELISAs and Western blots. Detects mouse IGF-I R in Immunohistochemistry. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha , rhIL-9 R, and rhTGF-beta RII.

Clone

33255

Applications

Western Blot, Flow Cytometry, Immunohistochemistry, Neutralization, ELISA

Conjugate

Unconjugated

Regulatory Status

RUO

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias

CD221, CD221 antigen, EC 2.7.10, EC 2.7.10.1, IGF1R, IGF-1R, IGF-I receptor, IGFIR, IGF-IR, IGFR, insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, JTK13, MGC142170, MGC142172, MGC18216, soluble IGF1R variant 1, soluble IGF1R variant 2

Gene Symbols

IGF1R

Isotype

IgG1

Purification Method

Protein A or G purified from hybridoma culture supernatant

Content And Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Description

  • IGF-I R/IGF1R Monoclonal specifically detects IGF-I R/IGF1R in Human, Mouse samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Neutralization, Immunocytochemistry, ELISA Capture (Matched Antibody Pair).