55-300-910T
MilliporeSigma™ anti-PhosphoDetect c-Raf (pTyr340, Tyr341), Polyclonal
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 55-300-910T-Each-of-1 | In Stock | ₹ 59,997.57 |
55-300-910T - Each of 1
In Stock
Quantity
1
Base Price: ₹ 59,997.57
GST (18%): ₹ 10,799.563
Total Price: ₹ 70,797.133
Antigen
PhosphoDetect™ c-Raf (pTyr340, Tyr341)
Classification
Polyclonal
Formulation
In phosphate buffer (without Mg2+and Ca2+), 1mg/mL BSA, 50% glycerol, pH 7.3 (+/- 0.1).
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr340and Tyr341, phosphorylation sites of human c-Raf (Raf-1)
Regulatory Status
RUO
Test Specificity
Recognizes c-Raf phosphorylated at Tyr340and Tyr341in HEK293 cells overexpressing c-Raf.
Content And Storage
−20°C
Isotype
IgG
Applications
Immunocytochemistry
Conjugate
Unconjugated
Host Species
Rabbit
Quantity
10 Tests
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Purified
Related Products
Description
- Specifically detects PhosphoDetect™ c-Raf (pTyr 340 , Tyr 341 ) Clone: in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry The raf family of proto-oncogenes consists of three members: A-raf-1, B-raf, and c-raf-1 which show the greatest degree of homology in the carboxy-terminal half of the molecule
- In the case of c-raf-1 this region has been shown to have protein (serine/threonine) kinase activity
- C-raf was first identified in an oncogeneic form, v-raf, which is the oncogene of the transforming virus MSV
- The raf-1 gene is the most studied of the three and encodes a protein with an apparent molecular mass of∽74kDa
- Raf is widely expressed and plays an important role in cell proliferation and differentiation
- Raf relays signals related to the activation of growth factor receptor to the activation of MAPK and utlimately to the nucleus
- Activation of raf itself is dependent on tyrosine (341) phosphorylation
- Tyrosine phosphorylation is accomplished by Src tyrosine kinase.