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5539173

Bromodeoxyuridine/BrdU Antibody (BRD.3), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Bromodeoxyuridine/BrdU

Concentration

0.2mg/mL

Applications

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Mouse, All species

Gene Alias

Bromodeoxyuridine, BUdr

Isotype

IgG1

Purification Method

Protein A or G purified

Test Specificity

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Clone

BRD.3

Dilution

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Immunogen

Bromodeoxyuridine (BrdU) conjugated to BSA

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Description

  • Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence Bromodeoxyuridine/BrdU Monoclonal specifically detects Bromodeoxyuridine/BrdU in All Species samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.