5539452

CD90/Thy1 Antibody (AF-9), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD90/Thy1

Concentration

0.2 mg/mL

Applications

Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Hematopoietic Stem Cell Markers, Hepatic Stem Cell Markers, Immunology, Innate Immunity, Mesenchymal Stem Cell Markers, Signal Transduction, Stem Cell Markers

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias

CD90, CD90 antigen, CDw90, FLJ33325, Thy-1 antigen, Thy-1 cell surface antigen, thy-1 membrane glycoprotein, Thy-1 T-cell antigen

Gene Symbols

THY1

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

Recognizes a protein of 18-35kDa, identified as CD90 (also known as Thy1). CD90 is a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids; 25-35 kDa) inserted into the cell membrane via a GPI anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblast and stromal cell lines, expressed on all thymocytes and peripheral T cells in mice, but in humans expressed only on small % fetal thymocytes, 10-40% of CD34+ cells in bone marrow, and <1% of CD3+CD4+ lymphocytes in peripheral circulation. It is also expressed by human lymph node HEV endothelium but not other endothelia. Lastly, it is expressed by a limited number of lymphoblastoid and leukemic cell lines.

Clone

AF-9

Dilution

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Bovine

Gene Accession No.

P04216

Gene ID (Entrez)

7070

Immunogen

Human T-lymphoma cells

Primary or Secondary

Primary

Content And Storage

Store at 4C.

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Description

  • Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence CD90/Thy1 Monoclonal specifically detects CD90/Thy1 in Human, Bovine samples
  • It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence.