PCNA Antibody (PCNA/694), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
PCNA
Concentration
0.2mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Autophagy, Base Excision Repair, Cell Cycle and Replication, Cellular Markers, Core ESC Like Genes, DNA Polymerases, DNA Repair, Stem Cell Markers
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias
cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
Gene Symbols
PCNA
Isotype
IgG2a κ
Purification Method
Protein A or G purified
Test Specificity
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
Clone
PCNA/694
Dilution
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Accession No.
P12004
Gene ID (Entrez)
5111
Immunogen
Recombinant full length human PCNA protein
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
36 kDa
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Description
- Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence PCNA Monoclonal specifically detects PCNA in Human samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.