5540251

S100A9 Antibody (S100A9/1011), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

S100A9

Concentration

0.2 mg/mL

Applications

Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, Immunology, Innate Immunity, Signal Transduction, Tyrosine Kinases

Formulation

10mM PBS with 0.05% Sodium Azide

Gene ID (Entrez)

6280

Immunogen

Recombinant human S100A9 protein

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

14 kDa

Clone

S100A9/1011

Dilution

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1 ug/ml, Immunohistochemistry-Frozen (Negative), Immunofluorescence 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Alias

CAGBMigration inhibitory factor-related protein 14, calgranulin B, calgranulin-B, Calprotectin L1H subunit, CFAGMRP-14,60B8AG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14Leukocyte L1 complex heavy chain, NIF, P14, protein S100-A9, S100 calcium binding protein A9, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B)

Gene Symbols

S100A9

Isotype

IgM κ

Purification Method

Protein A or G purified

Test Specificity

Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14); expressed by granulocytes, monocytes and by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cel

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Description

  • Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunohistochemistry (Frozen) (Negative) S100A9 Monoclonal specifically detects S100A9 in Human samples
  • It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.