MFG-E8 Antibody (SPM291), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
MFG-E8
Concentration
0.2mg/mL
Applications
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez)
4240
Immunogen
Human milk fat globule membrane preparation
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
45 kDa
Clone
SPM291
Dilution
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
BA46, Breast epithelial antigen BA46, EDIL1, hP47, HsT19888, lactadherin, medin, MFG1, MFG-E8, MFGM, Milk fat globule-EGF factor 8, milk fat globule-EGF factor 8 protein, O-acetyl disialoganglioside synthase, OAcGD3S, SED1, SPAG10, sperm associated antigen 10, sperm surface protein hP47
Gene Symbols
MFGE8
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
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Description
- Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence MFG-E8 Monoclonal specifically detects MFG-E8 in Human samples
- It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.