NuMA Antibody (SPM300), Novus Biologicals™

NuMA

0.2 mg/mL

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Unconjugated

Mouse

Breast Cancer, Cell Biology, Cell Cycle and Replication, Cellular Markers, Mitotic Regulators

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

4926

Colon carcinoma 174T cells

Primary

Store at 4C.

228 kDa

SPM300

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Monoclonal

Purified

RUO

Human

centrophilin stabilizes mitotic spindle in mitotic cells, nuclear mitotic apparatus protein 1, NUMA, NuMA protein, SP-H antigen, structural nuclear protein

NUMA1

IgM κ

Protein A or G purified

Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.