CD45RA Monoclonal Antibody (HI100), Alexa Fluor™ 700, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each | 56-045-842-Each | In Stock | ₹ 21,417.85 |
56-045-842 - Each
In Stock
Quantity
1
Base Price: ₹ 21,417.85
GST (18%): ₹ 3,855.213
Total Price: ₹ 25,273.063
Antigen
CD45RA
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.
P08575
Gene Symbols
PTPRC
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
5788
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
HI100
Conjugate
Alexa Fluor 700
Gene
PTPRC
Gene Alias
B220, CD45, CD45 antigen, CD45 antigen isoform 1 precursor, CD45 antigen isoform 2 precursor, CD45 antigen isoform 3 precursor, CD45 antigen isoform 4 precursor, CD45 antigen isoform 5 precursor, CD45 antigen isoform 6 precursor, CD45R, GP180, LCA, L-CA, leucocyte common antigen, Leukocyte common antigen, leukocyte common antigen A, leukocyte common antigen B, LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C, LY5, Ly-5, Lyt-4, protein tyrosine phosphatase receptor type C, protein tyrosine phosphatase, receptor type C, protein tyrosine phosphatase, receptor type, C, protein tyrosine phosphatase, receptor type, c polypeptide, Protein tyrosine phosphatase, receptor-type, c polypeptide, protein tyrosine phosphatase, alternatively spliced, PTPRC, Receptor-type tyrosine-protein phosphatase C, RT7, T100, T200, T200 glycoprotein, T200 leukocyte common antigen
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG2b κ
Related Products
Description
- Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells
- The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets
- Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This HI100 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.25 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
- Most instruments will require a 685 LP mirror and 710/20 band pass filter
- Please make sure that your instrument is capable of detecting this fluorochrome
- Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
- CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
- CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
- CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains
- CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
- Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
- CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues
- It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions
- Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.