CD11b Monoclonal Antibody (M1/70), Alexa Fluor™ 532, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 58-011-282-Each-of-1 | In Stock | ₹ 33,197.00 |
58-011-282 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 33,197.00
GST (18%): ₹ 5,975.46
Total Price: ₹ 39,172.46
Antigen
CD11b
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide, pH 7.2
Gene Accession No.
P05555
Gene Symbols
ITGAM
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
16409
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
M1/70
Conjugate
Alexa Fluor 532
Gene
ITGAM
Gene Alias
antigen CD11b (p170), antigen CD11b (p170), macrophage antigen alpha polypeptide, CD11 antigen-like family member B, CD11b, CD11B (p170), CD11b/CD18, cell surface glycoprotein MAC-1 alpha subunit, cell surface glycoprotein MAC-1 subunit alpha, complement component 3 receptor 3 subunit, complement component receptor 3 alpha-a, complement receptor type 3, CR3, CR-3 alpha chain, CR3A, F730045J24Rik, integrin alpha M, integrin alpha-M, integrin subunit alpha M, integrin, alpha M, integrin, alpha M (complement component 3 receptor 3 subunit), ITGAM, Leukocyte adhesion receptor MO1, leukocyte integrin alpha-M chain, LOC100351865, Ly-40, MAC1, Mac-1, Mac-1 alpha, Mac-1 alpha (Mac1A), MAC-1 alpha subunit, MAC1A, Mac-1a, macrophage antigen alpha, macrophage antigen alpha polypeptide, MGC117044, MO1A, Neutrophil adherence receptor, neutrophil adherence receptor alpha-M subunit, SLEB6, unnamed protein product
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2b κ
Related Products
Description
- Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM
- CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen
- Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity
- M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells
- Through interactions with its ligands, CD11b participates in adhesive cell interactions
- Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
- This may be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm
- This cannot be used with the Yellow-Green laser (561 nm)
- We recommend using a 560/14 band pass filter
- Please make sure that your instrument is capable of detecting this fluorochrome
- CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18)
- The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3)
- CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain
- CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions
- CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue
- The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages
- Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages
- Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.