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61-588-942

CD275 (B7-H2) Monoclonal Antibody (MIH12), PE-eFluor™ 610, eBioscience™, Invitrogen™

Manufacturer: eBioscience

Select a Size

Pack Size SKU Availability Price
Each of 1 61-588-942-Each-of-1 In Stock ₹ 34,265.00

61-588-942 - Each of 1

₹ 34,265.00

In Stock

Quantity

1

Base Price: ₹ 34,265.00

GST (18%): ₹ 6,167.70

Total Price: ₹ 40,432.70

Antigen

CD275 (B7-H2)

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

O75144

Gene Symbols

ICOSLG

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

23308

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

MIH12

Conjugate

PE-eFluor 610

Gene

ICOSLG

Gene Alias

AU044799; B7 homolog 2; B7 homologue 2; B7h; B7H2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP1; B7RP-1; BG071784; CD275; GI50; GL50; GL50-B; ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell costimulator ligand; inducible T-cell co-stimulator ligand; KIAA0653; LICOS; Ly115l; mKIAA0653; RGD1562791; transmembrane protein B7-H2 ICOS ligand

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

Description

  • Description: The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand
  • B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily
  • Human B7RP-1 is expressed by activated monocytes/macrophages
  • B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells
  • The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway
  • Applications Reported: This MIH12 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This MIH12 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood monocyte-derived dendritic cells
  • This may be used at 5 μL (0.125 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
  • We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red)
  •  Please make sure that your instrument is capable of detecting this fluorochrome
  • Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (Product No
  • Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS)
  • ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function
  • ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue
  • Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.