CD86 (B7-2) Monoclonal Antibody (IT2.2), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: The IT2.2 monoclonal antibody reacts with human CD86, an ∽80 kDa surface receptor also known as B7-2
• CD86 and CD80 are members of the B7 family of costimulatory molecules
• CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors
• In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4)
• The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production
• The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response
• Applications Reported: This IT2.2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This IT2.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• CD86 is one of two ligands (the other CD80) for CTLA4 and CD28
• CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation
• Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance
• CD80 appears to play a role distinct from CD80 in T helper cell differentiation
• CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily
• The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4
• Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell
• Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response
• Alternative splicing results in two transcript variants encoding different isoforms of CD86
• Additional transcript variants have been described for CD86, but their full-length sequences have not been determined
• Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.