CD18 (LFA-1 beta) Monoclonal Antibody (6.7), Super Bright™ 600, eBioscience™, Invitrogen™
Manufacturer: eBioscience
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 63-018-942-Each-of-1 | In Stock | ₹ 37,113.00 |
63-018-942 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,113.00
GST (18%): ₹ 6,680.34
Total Price: ₹ 43,793.34
Antigen
CD18 (LFA-1 beta)
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P05107
Gene Symbols
ITGB2
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
3689
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
6.7
Conjugate
Super Bright 600
Gene
ITGB2
Gene Alias
2E6; AI528527; antigen CD18; B-2 integrin; CD18; CD18 leukocyte adhesion molecule; CD18 subunit; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; complement receptor C3 subunit beta; integrin beta 2; integrin beta chain, beta 2; integrin beta-2; integrin subunit beta 2; integrin, beta 2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (antigen CD18 subunit (p95), lymphocyte function-associated antigen 1, integrin B2); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; Leu-CAM receptor; leukocyte cell adhesion molecule CD18; leukocyte surface protein; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; Lfa1; LFA-1; lymphocyte function associated antigen 1; MAC-1; Mac-1 beta; macrophage antigen 1 (mac-1) beta subunit); macrophage antigen-1 beta; MF17; MFI7
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The 6.7 monoclonal antibody reacts with human and non-human primate (rhesus or cynomologus) CD18, which is also known as beta2 integrin
- CD18 is a 90-95 kDa type I transmembrane protein that heterodimerizes with the a chain of CD11a, CD11b and CD11c
- The CD18/CD11a complex is also known as lymphocyte function-associated antigen-1 (LFA-1) and plays an important role in cellular adhesion and inflammatory reactions
- CD18 is expressed broadly by human lymphocytes and monocytes
- Peripheral blood granulocytes also express CD18, but at lower levels
- The 6.7 monoclonal antibody has been reported to induce the proliferation of resting T cells cultured with monocytes
- Applications Reported: This 6.7 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 6.7 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
- We recommend using a 610/20 bandpass filter
- Please make sure that your instrument is capable of detecting this fluorochrome
- CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes
- CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling
- CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18)
- In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts
- Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease
- CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis
- Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1)
- Two transcript variants encoding the CD18 protein have been identified.