CD62P (P-Selectin) Monoclonal Antibody (Psel.KO2.3), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• Description: The Psel.KO2.3 monoclonal antibody reacts with mouse CD62P (also known as P-selectin and GMP-140), a 140-kDa cell adhesion molecule that can be induced on endothelial cells and platelets
• During inflammation, this selectin mediates adhesion of monocytes and neutrophils to activated platelets, as well as leukocyte rolling on endothelial cells
• The major ligand for CD62P on neutrophils is P-selectin glycoprotein ligand-1 (PSGL-1)
• This monoclonal antibody recognizes an epitope that is conserved across several species (e.g
• mouse, human, and rat) and has not been reported to block cell adhesion
• Applications Reported: This Psel.KO2.3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This Psel.KO2.3 antibody has been tested by flow cytometric analysis of thrombin-activated platelets
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
• We recommend using a 610/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling
• P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets
• From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate
• P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes
• P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes
• P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction
• Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.