CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), Super Bright™ 702, eBioscience™, Invitrogen™

Description

• Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule
• CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha)
• A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha
• CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells
• Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm
• We recommend using a 710/50 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily
• CD7 contains N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects
• CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia
• Further, CD7 is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells
• Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation
• CD7 associates directly with phosphoinositol 3'-kinase
• CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation
• Expression of CD7 is an important marker used in leukemia diagnostics.