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70-174-6

Phospho-IRS1 (Ser312) Recombinant Rabbit Monoclonal Antibody (18H26L6), Invitrogen™

Manufacturer: Thermo Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 70-174-6-Each-of-1 In Stock ₹ 55,358.00

70-174-6 - Each of 1

₹ 55,358.00

In Stock

Quantity

1

Base Price: ₹ 55,358.00

GST (18%): ₹ 9,964.44

Total Price: ₹ 65,322.44

Antigen

Phospho-IRS1 (Ser312)

Classification

Recombinant Monoclonal

Concentration

0.5 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P35568

Gene Symbols

IRS1

Immunogen

Peptide corresponding to Human IRS1 (aa 309-318).

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG

Applications

Western Blot

Clone

18H26L6

Conjugate

Unconjugated

Gene

IRS1

Gene Alias

G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185

Host Species

Rabbit

Purification Method

Protein A

Regulatory Status

RUO

Gene ID (Entrez)

3667

Content And Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Form

Liquid

Description

  • This antibody is predicted to react with Monkey, Pig and Cat
  • Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
  • The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
  • Then, individual clones are screened to select the best candidates for production
  • The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
  • IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4)
  • IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues
  • An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo
  • Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II
  • Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade
  • IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.