Human SPARC Antibody, R&D Systems™
Mouse Monoclonal Antibody has been used in 5 publications
Manufacturer: Fischer Scientific
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Antigen
SPARC
Dilution
Immunohistochemistry 8-25 ug/mL, Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, CyTOF-ready
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias
Basement-membrane protein 40, BM-40, ONcysteine-rich protein, osteonectin, Secreted protein acidic and rich in cysteine, secreted protein, acidic, cysteine-rich (osteonectin)
Gene Symbols
SPARC
Isotype
IgG1
Purification Method
Protein A or G purified from hybridoma culture supernatant
Content And Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone
122511
Applications
Immunohistochemistry, Flow Cytometry, CyTOF
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human
Gene Accession No.
P09486
Gene ID (Entrez)
6678
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SPARC Ala18-Ile303 Accession # P09486
Primary or Secondary
Primary
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity
Detects human SPARC/Osteonectin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse SPARC/Osteonectin is observed.
Related Products
Description
- SPARC Monoclonal specifically detects SPARC in Human samples
- It is validated for ELISA, Immunohistochemistry, Intracellular Staining by Flow Cytometry, CyTOF-ready.