Ly-49I Monoclonal Antibody (YLI-90), FITC, eBioscience™, Invitrogen™

Description

• Description: The YLI-90 monoclonal antibody reacts with the B6 mouse Ly-49I receptor
• Ly49I, a member of the Ly49 family with inhibitory function, is a homodimer type II transmembrane protein and contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM)
• YLI-90 binds to subpopulations of NK, NKT and T cell lineages in the C57Bl/6 strain
• This antibody does not bind to cells from BALB/c, SJL or AKR/J mice
• Applications Reported: YLI-90 has been reported for use in flow cytometric analysis
• Applications Tested: The YLI-90 antibody has been tested by flow cytometric analysis of C57Bl/6 and BALB/c mouse splenocytes and can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated
• These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.