7068506
GARP Monoclonal Antibody (YGIC86), PE, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
GARP
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
AI426318; D11S833E; D11S833Eh; D7H11S833E; EG434215; Garp; garpin; Glycoprotein A repetitions predominant; leucine rich repeat containing 32; leucine-rich repeat-containing protein 32; Lrrc32; Transforming growth factor beta activator LRRC32
Gene Symbols
LRRC32
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
LRRC32
Clone
YGIC86
Applications
Flow Cytometry
Conjugate
PE
Host Species
Rat
Target Species
Mouse
Gene Accession No.
G3XA59
Gene ID (Entrez)
434215
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The YGIC86 monoclonal antibody reacts with mouse Glycoprotein A Repetitions Predomit (GARP, also known as Lrrc32 or Garpin)
- GARP is an approximately 80 kDa glycoprotein that is expressed on the cell surface
- Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas
- Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells
- The expression of GARP on Treg cells has been reported to be necessary for the suppressive function of Treg cells, possibly related to its role as a cell surface receptor for LAP/TGF beta
- When looking for expression of GARP on platelets, it is recommended to use the Foxp3 Staining Buffer Set (cat
- 00-5523) to fix the platelets before staining with this YGIC86 monoclonal antibody
- Fixation/permeabilization is not necessary when staining Treg cells
- YGIC86 does not cross-react to rat GARP
- Applications Reported: This YGIC86 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This YGIC86 antibody has been tested by flow cytometric analysis of stimulated mouse spleen cells
- This can be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region
- GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation
- Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.