7069508

MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

CAS Number: 28-14-8

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Antigen

MHC Class I (H-2Db)

Concentration

0.5 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein

Gene Symbols

H2-D1

Primary or Secondary

Primary

Content And Storage

4° C

Gene

H2-D1

Clone

28-14-8

Applications

Flow Cytometry

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Mouse

Gene Accession No.

P01895, P01896, P01897, P01899, P01900, P14426, P14427

Gene ID (Entrez)

14964

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q
  • Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin
  • Applications Reported: 28-14-8 has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, immunoprecipitation and complement-dependent cytotoxicity
  • Applications Tested: The 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 1 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Purity: Greater than 90%, as determined by SDS-PAGE
  • Aggregation: Less than 10%, as determined by HPLC
  • Filtration: 0.2 μm post-manufacturing filtered
  • This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8
  • HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.