Baculovirus Envelope gp64 Monoclonal Antibody (AcV1), eBioscience™, Invitrogen™

Description

• Description: The AcV1 monoclonal antibody reacts with the gp64 envelope protein of the baculovirus Autographa californica (AcMNPV)
• The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection
• The AcV1 antibody can be used in flow cytometry-based viral titration experiments
• Applications Reported: The AcV1 antibody has been reported for use in flow cytometric analysis
• This antibody can be used in identifying virally-infected insect cells
• Applications Tested: The AcV1 antibody has been tested by flow cytometry on baculovirus infected insect cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Baculoviruses are known to infect invertebrates, with over 600 species having been described
• Immature (larval) forms of moth species are the most common hosts, but these viruses have also been found infecting sawflies, mosquitoes, and shrimp
• Although baculoviruses are capable of entering mammalian cells in culture, they are not known to be capable of replication in mammalian or other vertebrate animal cells
• The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection.